Lentiviral shRNA library consortium

We are creating a viral-vector-based RNA interference (RNAi) library against mouse and human genes. Short hairpin RNAs (shRNAs) that generate siRNAs intracellularly are expressed from lentivirus and Moloney leukemia virus vectors, allowing screening in a wide range of mammalian cell types. The lentiviral vector permits infection of differentiated and non-dividing cells, such as neurons and dendritic cells.

The libraries will consist of bacterial glycerol stocks harboring sequence-verified shRNA Moloney and lentiviral vectors for mouse and human genes with 4-5 monoclonal stocks per gene. These libraries will be statistically or comprehensively validated for knockdown efficiency in mammalian cells. Methods for efficient screening with this library are being developed and will be shared. Our intent is that these libraries will be freely distributed to academic scientists and we plan on making subsets of the libraries available as they are produced.

The creation of the RNAi library is the joint effort of several research groups at the Whitehead Institute, the Broad Institute, the Dana Farber Cancer Institute, and the Massachusetts General Hospital.

Consortium Scientists
David Root, Project Leader, Broad Institute
Greg Hinkle, Dana Farber, Whitehead Institute
Biao Luo, Broad Institute
Chad Nusbaum, Broad Institute
Xiaoping Yang, Broad Institute

Founding Laboratories
Nir Hacohen, Massachusetts General Hospital
William Hahn, Dana Farber Cancer Institute
Eric Lander, Broad Institute
David Sabatini, Whitehead Institute, MIT
Sheila Stewart, Washington University
Brent Stockwell, Columbia University