Immunofluorescence Detection of Transfected Cell Arrays

Below is a laser scan image (5 µm resolution) of a large array expressing GFP (green, acquired under FITC filter) and HA-GST (red, acquired under Cy3 filter) in the pattern of WIBR (Whitehead Institute of Biomedical Research). The reason for the appearance of green specks in the red spots is that the very intense Cy3 signal bleeds through during the scanning using the FITC filter. The intensity of the Cy3 signal was intentionally decreased to make the WIBR stand out: