Detection of Protein Expression by Immunofluorescence and Scanning

The microarray below contains 432 clusters of transfected cells in four 12 X 9 subgrids. Cell clusters in rows 1, 4 and 7 express EGFP. Cell clusters in rows 2, 5 and 8 express HA-tagged-GST and cell clusters in rows 3, 6 and 9 express V5-tagged PDGF receptor. HA-tagged and V5-tagged proteins were labeled via anti-HA and anti-V5 immunofluorescence, respectively, using a Cy3-labeled secondary antibody. Fluorophores were detected by sequential laser scanning for FITC and Cy3 signals , the images pseudocolored and superimposed using Adobe Photoshop 5.5.

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